Page 137 - Applied Pharmacognosy
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Basic Policy for Selecting Separation Mode
Reversed phase mode is the first choice
❑ Reversed phase mode using an ODS column is the first
choice!
❑ Exceptions include:
➢ Large molecular weight (> 2,000) Size exclusion
➢ Optical isomers Chiral column
➢ Positional isomers Normal phase
➢ Inorganic ions Ion chromatography
➢ Sugars, amino acids, Special column
➢ short-chain fatty acids
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