Page 142 - Applied Pharmacognosy
P. 142
Mobile Phase Selection
Guidelines for Setting Mobile Phase Conditions
❑ If the sample is cationic i.e. basic:
Reversed phase mode is still an option…. But
❑ Mobile Phase Strength has to be adjusted by changing Eluent
Composition to be either:
➢ Basic buffer solution / acetonitrile. What is the
➢ Basic buffer solution / methanol. problem??
❑ This method is certainly effective with regard to separation,
but it creates another problem and is not often used.
Increase retention strength by making the eluent alkaline
and suppressing ionization! 141
