Page 138 - Applied Pharmacognosy
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Mobile Phase Selection
Guidelines for Setting Mobile Phase Conditions
❑ The first case scenario; if sample is Neutral (Nonionic) and not
any of the previously mentioned exceptions:
Reversed phase mode is the Optimal
Choice
❑ Eluent Composition: With neutral substances, pH of the
➢ Water / acetonitrile. eluent has almost no influence on
➢ Water / methanol.
the retention strength
❑ Separation Adjustment:
➢ Changing the mixing ratio of the water and organic solvent.
➢ Changing the type of organic solvent.
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