Page 136 - Applied Pharmacognosy
P. 136
Mobile Phase Selection
How to adjust the Mobile phase Strength?
❑ Mobile Phase Strength is 100/0
adjusted by changing: 98/2
➢ Type of Modifier (MeOH,
ACN).
➢ Solvent Strength (%
modifier), has a greater
influence on separation.
Eluent: Hexane/methanol
As polarity of eluent 95/5
Overall retention time
Normal Phase Separation 135
