Page 129 - Applied Pharmacognosy
P. 129
Differences in Solute Retention Strengths for Water
and Water-Soluble Organic Solvents
Reversed Phase
❑ The greater the proportion of water in the eluent, the greater
the solute retention strength.
Tightly packed network Loose network
H2O H2O CH3OH CH3OH
H2O H2O CH3OH CH3OH CH3OH
H2O H2O H2O
CH3OH Nonpolar solute CH3OH
Nonpolar solute
Nonpolar stationary phase 128
