Page 198 - Applied Pharmacognosy

Data handling-Chromatogram Information

❑ A chromatogram is a graph showing the detector response as a function of
retention time.

❑ In a chromatogram chart the number of sample peaks indicates the number
of components present in the sample.

❑ The retention time, tR, of every peak is used in identifying this component
when compared with a reference sample chromatographed under identical
condition.

❑ The area under a peak is used in quantitative analysis.

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