Page 33 - Applied Pharmacognosy

CHROMATOGRAPHY-Basic Concepts

Art & Science of Separation

❑ Chromatography is a physical process by which mixtures can

be resolved into their individual components.
❑ In all chromatographic separations, the sample solution is

introduced into 2-phase system (stationary phase or sorbent

and mobile phase or Degree of interaction Sample mixture
developer). forces (adsorption, (Solute)
solubility, ionicity, etc).

Differences in the interactions between Stationary Mobile
the solute components and stationary phase phase
and mobile phases enable separation
(Differential Migration) in the form of Chromatographic Sys32tem
bands or spots.