Page 34 - Applied Pharmacognosy
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CHROMATOGRAPHY-Basic Concepts
Art & Science of Separation
❑ Chromatographic separation depends on Physical Characters.
❑ Importantly, solutes are separated by a dynamic differential
migration in a system of two phases, one phase is held in
place while the other moves continuously past it.
Degree of interaction Sample mixture
forces (adsorption, (Solute)
solubility, ionicity, etc).
Stationary Mobile
phase phase
Chromatographic Sys33tem
