Page 38 - Applied Pharmacognosy
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CHROMATOGRAPHY-Basic Concepts

Solvent

Plant
extract

Stationary        Column                  Sample mixture
  phase     Chromatography                      (Solute)

   Column
 Piece of
 cotton

• Eluent: Fluid entering the column.      Stationary  Mobile
• Eluate: Fluid exiting the column.          phase    phase
• Elution: The process of passing liquid
                                          Chromatographic Sys3t7em
  or gas through a column.
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