Page 76 - Applied Pharmacognosy
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Evolution of HPLC
❑ In traditional column technology, particle size of the
stationary phase was fairly large (>100 µm).
❑ Looking for higher resolution, smaller particle size
stationary phase packing material was developed (<10
µm).
❑ Fine column packing (3-50 μm) required high pressure
pumping systems capable of pushing the mobile
phase through the column at pressures up to 6000 psi
to achieve appropriate flow rates.
High-performance Liquid Chromatography 75
