Page 79 - Applied Pharmacognosy
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What is HPLC used for???
Separation and Analysis
❑ Separation of mixed components.
Small molecules Macro-molecules Ionic substances
❑ Qualitative analysis / Quantitative analysis.
❑ Purity and Stability testing.
❑ Preparation of interest components.
Ease of sample HPLC
A AA A recovery 78
C BB
A C Separation
BAB C CCC
SampC le mCixture
(Solute) Qualitative analysis
What are components A, B and C ?
Quantitative analysis
What is the concentration of
components A, B and C ?