Page 79 - Applied Pharmacognosy
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What is HPLC used for???

                    Separation and Analysis

❑ Separation of mixed components.

Small molecules Macro-molecules Ionic substances

❑ Qualitative analysis / Quantitative analysis.

❑ Purity and Stability testing.

❑ Preparation of interest components.

                                            Ease of sample  HPLC

A                       AA A                     recovery           78
   C                    BB
      A  C  Separation

BAB                     C CCC

SampC le mCixture
(Solute) Qualitative analysis
                   What are components A, B and C ?

            Quantitative analysis

                   What is the concentration of

                   components A, B and C ?
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