Page 78 - Applied Pharmacognosy

Evolution of HPLC

Impact of Particle Size Reduction on Resolution

NOW, It is time for UPLC!!!

❑ In 2004, further advances in instrumentation and column
technology resulted in very significant increase in Resolution,
Speed and Sensitivity of Liquid Chromatography.

What the future will provide; research HPLC UPLC
being carried out with columns containing

1 micron particles, and instrumentation
capable of performing at 100,000psi. !!!!!!

. Particle size μ Pressure

Column Chromatography < 10 > 20 bar, > 500 psi
1.7 15,000 psi 77
High Pressure LC
Ultra Pressure LC