Page 97 - Applied Pharmacognosy

Sample Management

Sample is Everything

❑ Sample properties will guide the choice of HPLC parameters.
✓ Nature (Polar or non polar).
✓ Complexity (Mixture components and their chemistry).
✓ Functional groups (acidic or basic groups).
✓ Solubility.
✓ Purity (particles free & permissible adsorbed substances).
✓ Molecular weight.
✓ Detection.

Sample treatment Sample volume

Sample size 96