Page 130 - BIOCHEMISTRY II

Uses of NADPH

IJ. Reductive biosynthesis
NADPH is a high energy molecule and electron donor. It is
required as a source of electrons for biosynthesis of FA,
cholesterol, sterols, hormones, and bile salts.

Ĵ. Reduction of H2O2: ROS-damage DNA, proteins, unsaturated
lipids– reperfusion injuries, cancer, inflammatory diseases,
aging.

Enzymes that catalyze Antioxidant reactions

Glutathione peroxidase or GOD [using reduced Glutathione or
GSH which is active as antioxidant, present in most cells which
can detoxify H2O2 ].

Glutathione reductase or GR [regenerates GSH from oxidized
glutathione formed in the above reaction] using NADPH as a
source of electrons.

132