Page 28 - Instrumental Analysis - Pharm D Clinical- 07-PA403

UV-visible Spectrophotometry

2- Double-beam spectrophotometer

• More technically advanced.
• Used primarily for research.

Errors in Spectrophotometry 51
Deviations from Beer’s Law 52

1- Fundamental “real” deviation
2- Instrumental deviations

I- Error in wavelength scale
II- Stray light effect

3- Chemical deviations

27