Page 44 - MNU PM502 Ph. Micreo practical

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

Equipments:

1. Two flasks, one containing 100 ml of 5% phenol (Standard Disinfectant) and the

other one containing 100 ml of 2% test disinfectant.

2. Flask containing sterile distilled water.

3. Flask containing inoculated yeast suspension (2ml of 24h broth culture + 48ml

of 3% yeast suspension). Yeast is used as a source of organic matter.

4. 4 tubes (sterile & empty).

5. 4 n. broth tubes (containing 10 ml).

6. 10 ml pipette.

7. Loop.

Steps:

1- Preparation of four different dilutions of both the phenol & the test

disinfectant in the four empty sterile test tubes Ex: for phenol:

Take 10ml from phenol flask to first empty test tube transfer 2.5ml & reject 7.5

ml. so after this step the first empty test tube will contain 2.5ml of 5%phenol and

the phenol flask will contain 90 ml of 5% phenol.

Then, transfer 10ml sterile distilled water to the flask of phenol so its

concentration will be 4.5%.

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