Page 71 - MNU PM502 Ph. Micreo practical

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

Microbial Enumeration Test

Microbial enumeration test is a quantitative test which

determines the Total Aerobic Microbial Count (TAMC) and

Total Yeast and Mold Count (TYMC) present in the test product.

• Total Aerobic Microbial Count (TAMC): Number of

colony forming units (cfu) found using Tryptic Soy Agar (TSA).

• Total Combined Yeasts and Molds Count (TYMC): Number of cfu found

using Sabouraud- Dextrose Agar (SDA).

• The total viable aerobic count is the sum of the bacterial count and the fungal

count

Microbial Growth Agar Media

Soybean-Casein Digest Agar- for TAMC

Sabouraud’s Dextrose Agar - for TYMC

• The test product is dissolved or diluted (1:10 dilution) in Phosphate Buffer

Solution (pH 7.2) or Soybean–Casein Digest Broth.

• A surface-active agent (such as polysorbate 80 may be added to assist the

suspension of poorly wettable substances.

Techniques

1-Plate Count methods

• Pour-Plate Method

• Surface-Spread Method

2- Membrane Filtration method: Uses filtration apparatus

• Plate Count Methods— Perform plate count methods at least in duplicate for each
medium and use the mean count of the result.

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