Page 73 - MNU PM502 Ph. Micreo practical
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Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)
8. Leave to solidify
9. Incubate the plates in inverted position at ted at 30–35 °c and 5 days for
bacteria and 20–25 °c, 7 days for
fungi
10. Count the number of colonies.
11. Plates corresponding to a single
dilution showing the highest
number of colonies less than 300
(100 for fungi) were selected for
counting.
12. Take the average of the numbers in the two plates of each medium
13. Calculate
/
TAMC: Number of CFU ml or gm found using Soybean-Casein Digest Agar.
TYMC: Number of CFU/ml or gm found using Sabouraud-Dextrose Agar.
60
