Page 95 - MNU PM502 Ph. Micreo practical

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

Antibiotic dilution chart for agar dilution method

• Adjust if a different concentration range is needed.

• Label agar flasks and petri dishes with the final antibiotic concentration

• With an automatic pipette dispense appropriate amounts of antibiotic solution

to the corresponding agar flask.

• mix well (avoid bubbles) and pour the 25 ml into a petri dish labeled with the

respective antibiotic concentration

• pour a control agar plate without any antibiotic. allow agar to solidify.

• Dry the surface of the agar plates either in an incubator or in a laminar air flow

hood for 30 min.

• Mark the bottom of the agar plates to define an orientation.

• Mix the bacterial suspension, adjusted to 0.5 MacFarland for each isolate.

• The inocula can be transferred to the agar plates using a 48-pin replicator (1µl

per spot) or by multichannel pipette.

• Make sure that each agar plate has the same orientation while inoculating and

to make a note of the orientation so that spots on the agar plate can be assigned

to the respective isolate tested.

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