Page 98 - MNU PM502 Ph. Micreo practical

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

LAB 9

Assay of Antibiotic by agar diffusion method

Aim of the work:

To determine the relative potency of a test antibiotic compared to a standard one.

Equipments:

• 2 empty sterile test tubes

• Test tube (S) containing 4 ml of standard antibiotic

• Test tube (T) containing 4 ml of test antibiotic

• Flask containing 50 ml of melted nutrient agar.

• Test tube containing sterile water or saline

• 2 pipettes 5 ml & 1 pipette 1ml

• 2 sterile Petri dishes

• 2 sterile Pasteur pipettes

• Loop

• Wasserman tube containing test microorganism

Steps:

I- Preparation of Antibiotics’ Dilutions: (2-fold serial dilution of the standard

& the test antibiotic)

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