Page 175 - MNU-PM502- Pharmaeutical Microbiology Theoritical Book
P. 175
Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)
Molecular Methods
PCR
• Molecular AST directly detects specific resistance genes, as well as
mutations in and expression of these genes
• Examples include: - mecA gene detection by PCR denotes resistance to
methicillin in Staph. aureus (MRSA).
• Antiviral drug resistance due to genetic point mutations can be examined by
PCR for HIV, CMV & HCV.
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