Page 33 - MNU-PM503 Parasitology practical book

Pharm D- Clinical Pharmacy Program Third Level Parasitology and virology (PM503)

Feature S. mansoni S. Haematobium

Geographic Africa and America Prevalent in Africa

distribution
Site of localization Superior mesenteric veins Venous plexus of bladder

draining the large intestine

Egg (diagnostic stage) Lateral spine Terminal spine
Intermediate Host Biomphalaria and Bulinus (Physopsis)

Australorbis

Definitive Host Man Man
Infective stage Cercaria Cercaria

Laboratory diagnosis

1- Microscopic examination of eggs in stool (S. Mansoni) or in urine (S.

haematobium)

Since eggs may be passed in small amounts their detection may enhanced by

repeated examination or concentration procedures (formaline ethylacetate technique)

2- Kato Katz Technique:

– For enumeration of eggs

– Most commonly used for evaluating epidemiology, effect of control measures,

drug trials

3- Immunodiagnosis

– Indirect hemagglutination using adult worm and egg antigens

– Enzyme-Linked Immunosorbent Assay (ELISA) using soluble antigens of

adults and eggs.

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