Page 173 - MNU microbiology theoritical 2025

General Microbiology & Immunology (PM 401) Second level Semester 4 2024/2025

⮚ DNA by polymerase I (exonuclease): remove RNA primers and replace

with newly synthesized DNA

⮚ DNA ligase: seals the gaps between Okazaki fragments with a

phosphodiester bond

Summary of DNA Replication

The DNA replication in prokaryotes takes place in the

following place:

1. The two strands of DNA unwind at the origin of

replication.

2. Helicase opens the DNA and replication forks are formed.

3. DNA is coated by the single-strand binding proteins around the

replication fork to prevent rewinding of DNA.

General Microbiology & Immunology (PM 401) theoretical handbook Page 162