Page 77 - MNU microbiology theoritical 2025
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General Microbiology & Immunology (PM 401)         Second level                Semester 4                    2024/2025


































                  2. Metabolic activity

                  -  Products of microbial growth can be used to estimate the number of

                      microorganisms or, more commonly, to confirm their presence.

                  -  This measures either:

                      o  One of the products of metabolism, e.g acid in glucose fermentation

                      o  the amount of substance used, e.g oxygen or enzyme, ATP

                  3. Total weight
                      •  Either dry or wet weight

                      •  The method is used to measure growth of filamentous organisms. These do

                         not readily separate into the individual cells necessary for a valid plate count


                      •  Disadvantages:       Tedious and time-consuming method












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