Page 12 - MNUmicrobiology practical 2025

General Microbiology & Immunology (PM 401) second level Semester 4 2024/2025

Course content

Title Page

• Aim of the course 2

• Microbiology lab safety rules 3
• Common equipment used in routine microbiology laboratory

• Introduction to the microscope
• Bacterial shapes, arrangements, and forms 16

• Examination of different bacterial shapes using light microscopes
• Inoculation and other aseptic procedures

• Preparation of bacterial smear 30
• Simple stain

• Gram stain 41

• Gram stain for single & mixed culture 63

• Acid Fast stain (Ziehl Neelsen Stain) 60

• Spore stain 68

• Aseptic Technique and Streak Plates 78
• Media in common use 86

• Biochemical activity of microorganism (part 1) 98

• Biochemical activity of microorganism (part 2)

• Fungi 113

• Serological test 119

General Microbiology & Immunology (PM 401) Practical note Page 1