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Genetic diversity of the pathogenic fungus Sarocladium oryzae
causing sheath rot on rice using rep-PCR

1
S Pramunadipta , A Widiastuti , A Wibowo , A Priyatmojo
2
2
2
1 Graduate Student of Doctoral Program in Agricultural Science, Faculty of
Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
2 Department of Crop Protection, Faculty of Agriculture, Universitas Gadjah Mada.
Jl. Flora No. 1, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia
E-mail: priyatmojo@ugm.ac.id

Abstract. Rice becomes the main commodity cultivated in Asia. Sheath rot disease becomes
one of the challenges in increasing rice production. Sarocladium oryzae is known to be one of
the major pathogens in causing sheath rot disease. This pathogen has been reported in several
rice production countries, such as Brazil, India, Taiwan, Philippines, Thailand, including
Indonesia, and causing yield loss of up to 85%. The study of genetic diversity is important to
investigate the rapid shift in genetic structures. A total of six S. oryzae strains showed diverse
in molecular using repetitive-PCR (rep-PCR). The isolate SO8 and SO13 isolate SO2 and SO11
and isolate SO3, and SO5 has the relatively same the band patterns in BOX, ERIC, and REP
sets of primers. The results of the UPGMA analysis show that S. oryzae in the various primers
can be divided into three different group consistency. This result indicates that the rep-PCR
method can genetically distinguish S. oryzae. The use of the three main sets, BOX, ERIC, and
REP, suggested differentiating the other fungal species.

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