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How is the COVID-19 Virus




          Detected using Real-Time RT-PCR?

























































                s the coronavirus that causes the COVID-19 disease  technique, how it works and a few refresher details on
                spreads across the world, the IAEA, in partnership  viruses and genetics
        Awith the Food and Agriculture Organization of the
                                                                 What is real-time RT–PCR?
        United Nations (FAO), is offering its support and expertise
        to help countries use real-time reverse transcription-   Real-time RT–PCR is a nuclear-derived method for
        polymerase chain reaction (real-time RT–PCR), one of the   detecting the presence of  specific genetic material in
        most accurate laboratory methods for detecting, tracking   any pathogen, including a virus. Originally, the method
        and studying the COVID-19 coronavirus.                 used radioactive isotope markers to detect targeted
                                                               genetic materials, but subsequent refining has led to the
          But what is real-time RT–PCR? How does it work? How
                                                               replacement of isotopic labelling with special markers,
        is it different from PCR? And what does this have to do
                                                               most frequently fluorescent dyes. This technique allows
        with nuclear technology? Here’s a handy overview of the
                                                               scientists to see the results almost immediately while the
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    152                                                                                 Techno India Group Public School
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