Page 22 - Practical Handout bio 2 mnu 2025_Neat
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Level two – Semester 4 - PharmD Biochemistry-II PB-403 (Practical course) 2024/2025
Sample Hemolysis
Hemolysis releases the cell contents into plasma and, consequently, the
concentrations of some plasma constituents, such as potassium and phosphate
may be falsely increased
.
:
1. Avoidable causes of hemolysis
a) Squeezing or intense pressure on fingertips or heel during capillary
collection
.
b) Retaining tourniquet on arm for longer than 1 minute
.
c) Failure to separate serum or plasma from cells within 1 hour of collection
.
d) Shaking or vigorous inversion of the blood sample.
2. Unavoidable causes of hemolysis:
1. Intravascular hemolysis can result from disorders, such as hemolytic anemia
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