Page 341 - 2014 Printable Abstract Book
P. 341
matching GI histopathology including H&E staining and crypts survival are being integrated with the
identified metabolite biomarkers to provide a more comprehensive understanding of radiation-induced
injuries. This work was supported by NIAID contract HHSN272201000046C.
(PS6-27) Using MALDI-MSI to enable biomarker identification and medical countermeasure
development for radiation-induced lung injury. Claire L. Carter, PhD; Jace W. Jones; Isabel L. Jackson;
Zeljko Vujaskovic; Stephanie Tabisz; Sean Kearney; Kory Barrow; Allison Gibbs; Anne M. Farese; Thomas
J. MacVittie; and Maureen Kane, University of Maryland, Baltimore, MD
Radiation-induced lung injury is a recognized delayed effect of acute radiation exposure (DEARE),
characterized by sub-acute pneumonitis and the later chronic onset of fibrosis, which can lead to
respiratory failure and mortality. The need for a clearer understanding of the mechanisms of action (MoA)
by which radiation induces tissue/organ damage and medical countermeasures (MCM) for treatment or
mitigation in the event of accidental or intentional radiation exposures is evident. The Medical
Countermeasures Against Radiological Threats (MCART) consortium has developed non-human primate
and murine models to study radiation-induced lung injury and to evaluate the therapeutic efficacy of
MCMs. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a unique
analytical technique that aids in the characterization of radiation-induced injury through its ability to
simultaneously detect and localize hundreds of molecules from an organ section or biopsy, in a single
experiment, without the need of a label or a predefined target. In addition to the utility of MALDI-MSI in
MoA and biomarker investigations, mass spectrometry imaging also has the ability to detect and localize
the accumulation of drugs and their metabolites within anatomical regions of target organs. As such,
MALDI-MSI was applied to DEARE models, with and without a potential MCM, AEOL 10150, to map the
molecular changes within lung sections following radiation. Preliminary data of naïve and irradiated lung
tissue demonstrates significant changes in the abundance and localization of numerous molecules. Images
of treated tissue show localization of AEOL10150 within anatomical regions of lung tissue. Investigations
were combined with liquid chromatography - tandem mass spectrometry (LC-MS/MS) for targeted
quantification, and histology for pathological classification. This work was supported by NIAID contract
HHSN272201000046C and Aeolus/BARDA contract HHSO0100201100007C.
(PS6-28) A novel method to estimate radiation dose to the lens of the eye from the CT dose index for
neuroradiology CT protocols in pediatric patients. Natalie Januzis; Giao Nguyen; Donald Frush; Jenny
Hoang; Carolyn Lowry; and Terry Yoshizumi, Duke University, Durham, NC
New lower thresholds for cataract formation (ICRP) justify the investigation of lens doses in
patients, particularly in children, from CT imaging of the head. The purpose of this study was two-fold: (1)
to measure the radiation dose to the lens of the eye for pediatric neuroradiology protocols on CT scanners,
and (2) to correlate lens doses to scanner-reported volumetric CT dose index (CTDIvol) values. Lens of the
eye radiation dose was measured by scanning three pediatric anthropomorphic phantoms (CIRS) of
varying ages (5 year old, 1 year old, and newborn) with MOSFET detectors. The phantoms were scanned
with current neuroradiology protocols that covered the orbits. All measurements were performed on 4
different scanners, including three 64-slice MDCT scanners and one 320-slice CT scanner, from three
different vendors. Linear regression analysis was used to determine the relationship of average lens dose
339 | P a g e
identified metabolite biomarkers to provide a more comprehensive understanding of radiation-induced
injuries. This work was supported by NIAID contract HHSN272201000046C.
(PS6-27) Using MALDI-MSI to enable biomarker identification and medical countermeasure
development for radiation-induced lung injury. Claire L. Carter, PhD; Jace W. Jones; Isabel L. Jackson;
Zeljko Vujaskovic; Stephanie Tabisz; Sean Kearney; Kory Barrow; Allison Gibbs; Anne M. Farese; Thomas
J. MacVittie; and Maureen Kane, University of Maryland, Baltimore, MD
Radiation-induced lung injury is a recognized delayed effect of acute radiation exposure (DEARE),
characterized by sub-acute pneumonitis and the later chronic onset of fibrosis, which can lead to
respiratory failure and mortality. The need for a clearer understanding of the mechanisms of action (MoA)
by which radiation induces tissue/organ damage and medical countermeasures (MCM) for treatment or
mitigation in the event of accidental or intentional radiation exposures is evident. The Medical
Countermeasures Against Radiological Threats (MCART) consortium has developed non-human primate
and murine models to study radiation-induced lung injury and to evaluate the therapeutic efficacy of
MCMs. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a unique
analytical technique that aids in the characterization of radiation-induced injury through its ability to
simultaneously detect and localize hundreds of molecules from an organ section or biopsy, in a single
experiment, without the need of a label or a predefined target. In addition to the utility of MALDI-MSI in
MoA and biomarker investigations, mass spectrometry imaging also has the ability to detect and localize
the accumulation of drugs and their metabolites within anatomical regions of target organs. As such,
MALDI-MSI was applied to DEARE models, with and without a potential MCM, AEOL 10150, to map the
molecular changes within lung sections following radiation. Preliminary data of naïve and irradiated lung
tissue demonstrates significant changes in the abundance and localization of numerous molecules. Images
of treated tissue show localization of AEOL10150 within anatomical regions of lung tissue. Investigations
were combined with liquid chromatography - tandem mass spectrometry (LC-MS/MS) for targeted
quantification, and histology for pathological classification. This work was supported by NIAID contract
HHSN272201000046C and Aeolus/BARDA contract HHSO0100201100007C.
(PS6-28) A novel method to estimate radiation dose to the lens of the eye from the CT dose index for
neuroradiology CT protocols in pediatric patients. Natalie Januzis; Giao Nguyen; Donald Frush; Jenny
Hoang; Carolyn Lowry; and Terry Yoshizumi, Duke University, Durham, NC
New lower thresholds for cataract formation (ICRP) justify the investigation of lens doses in
patients, particularly in children, from CT imaging of the head. The purpose of this study was two-fold: (1)
to measure the radiation dose to the lens of the eye for pediatric neuroradiology protocols on CT scanners,
and (2) to correlate lens doses to scanner-reported volumetric CT dose index (CTDIvol) values. Lens of the
eye radiation dose was measured by scanning three pediatric anthropomorphic phantoms (CIRS) of
varying ages (5 year old, 1 year old, and newborn) with MOSFET detectors. The phantoms were scanned
with current neuroradiology protocols that covered the orbits. All measurements were performed on 4
different scanners, including three 64-slice MDCT scanners and one 320-slice CT scanner, from three
different vendors. Linear regression analysis was used to determine the relationship of average lens dose
339 | P a g e
