Page 343 - 2014 Printable Abstract Book
P. 343
(PS6-30) Evaluating the comparative effectiveness of biodosimetry for intended uses by the military
and public health. Ann B. Flood, PhD; EPR Center for the Study of Viable Systems at Dartmouth,
Hanover, NH
The comparative effectiveness of biodosimetric methods for triaging a large number of people
following an unexpected radiation event depends on the aims of triage, the capacity of the biodosimetry
to provide timely, accurate information to triage decision-makers, and the feasibility of providing the
information. This paper contrasts the comparative effectiveness of biodosimetry methods in two
contexts: (1) triage of military forces for return to active duty or not, in the context of a terrorist or other
military threat versus (2) triage of a large civilian population for entry into the healthcare system or
not. Differences in aims for the military versus civilians include a greater need for maintaining an active
and effective force; a greater likelihood of having higher rates of significant exposures, inhomogeneous
exposures, significant doses from neutrons, and combined injury; and a need to assess a less diverse,
healthier population. The capabilities of the military versus the capabilities for responding to a civilian
event also differ, including a greater capacity to organize staff and facilities to carry out sophisticated field
deployed activities to assess dose, and an ability to respond more quickly to an event. Thus, selecting the
most effective biodosimetry methods for military versus civilian uses should take these differences into
account. As a consequence, the most appropriate biodosimetry techniques for military purposes will
include features that are less important for civilian settings such as being able to sample, analyze and
report the results to decision makers within hours following an event, needing to resolve whether the
exposure was homogeneous or heterogeneous, needing to determine whether neutrons contributed
significantly to dose, and dealing with significant concomitant injuries from blasts or burns. These differing
goals, capacities and scenarios lead to different conclusions about which biodosimetric methods are the
most appropriate to plan for the most effective preparations of the staff, supplies and facilities needed
for the response, the level of training of responders, and the strategies for deployment.
1
(PS6-31) The use of biotech robotic systems for automation of biodosimetry assays. Mikhail Repin ;
1
1
2
1
Sergey Pampoy ; Helen C. Turner ; Guy Garty ; and David J. Brenner , Center for High-Throughput
1
Minimally Invasive Radiation Biodosimetry, Columbia University Medical Center, New York, NY and
Columbia University, New York, NY
2
For fast and efficient response to radiation events involving mass casualties, it has become
imperative to automate well-established biodosimetry assays. In the USA there are many commercial,
high-throughput biotech robotic systems that can potentially be used for the rapid radiation dose
estimation using different biological dosimetry assays. We propose the combined use of plates and tubes
in racks compatible with ANSI/SLAS microplate formats as platforms of next generation for high
throughput radiation biomarker analysis. Here, we demonstrate the use of these platforms at all stages
of standard cytogenetic assay protocols starting from blood collection into microtubes organized in
standardized racks and ending with the image analysis of samples in standardized multiwell and
multichannel plates. The introduction of next generation platforms in biodosimetry will allow for high
throughput and cost-effective assay automation in many readily accessible systems. In the present work,
we have utilized the commercial Cell::Explorer universal robot (Perkin Elmer) at Columbia Genome Center
to develope and optimize automated protocols for the dicentric and cytokinesis-block micronucleus
assays. Work supported by NIAID grant 5 U19-AI067773.
341 | P a g e
and public health. Ann B. Flood, PhD; EPR Center for the Study of Viable Systems at Dartmouth,
Hanover, NH
The comparative effectiveness of biodosimetric methods for triaging a large number of people
following an unexpected radiation event depends on the aims of triage, the capacity of the biodosimetry
to provide timely, accurate information to triage decision-makers, and the feasibility of providing the
information. This paper contrasts the comparative effectiveness of biodosimetry methods in two
contexts: (1) triage of military forces for return to active duty or not, in the context of a terrorist or other
military threat versus (2) triage of a large civilian population for entry into the healthcare system or
not. Differences in aims for the military versus civilians include a greater need for maintaining an active
and effective force; a greater likelihood of having higher rates of significant exposures, inhomogeneous
exposures, significant doses from neutrons, and combined injury; and a need to assess a less diverse,
healthier population. The capabilities of the military versus the capabilities for responding to a civilian
event also differ, including a greater capacity to organize staff and facilities to carry out sophisticated field
deployed activities to assess dose, and an ability to respond more quickly to an event. Thus, selecting the
most effective biodosimetry methods for military versus civilian uses should take these differences into
account. As a consequence, the most appropriate biodosimetry techniques for military purposes will
include features that are less important for civilian settings such as being able to sample, analyze and
report the results to decision makers within hours following an event, needing to resolve whether the
exposure was homogeneous or heterogeneous, needing to determine whether neutrons contributed
significantly to dose, and dealing with significant concomitant injuries from blasts or burns. These differing
goals, capacities and scenarios lead to different conclusions about which biodosimetric methods are the
most appropriate to plan for the most effective preparations of the staff, supplies and facilities needed
for the response, the level of training of responders, and the strategies for deployment.
1
(PS6-31) The use of biotech robotic systems for automation of biodosimetry assays. Mikhail Repin ;
1
1
2
1
Sergey Pampoy ; Helen C. Turner ; Guy Garty ; and David J. Brenner , Center for High-Throughput
1
Minimally Invasive Radiation Biodosimetry, Columbia University Medical Center, New York, NY and
Columbia University, New York, NY
2
For fast and efficient response to radiation events involving mass casualties, it has become
imperative to automate well-established biodosimetry assays. In the USA there are many commercial,
high-throughput biotech robotic systems that can potentially be used for the rapid radiation dose
estimation using different biological dosimetry assays. We propose the combined use of plates and tubes
in racks compatible with ANSI/SLAS microplate formats as platforms of next generation for high
throughput radiation biomarker analysis. Here, we demonstrate the use of these platforms at all stages
of standard cytogenetic assay protocols starting from blood collection into microtubes organized in
standardized racks and ending with the image analysis of samples in standardized multiwell and
multichannel plates. The introduction of next generation platforms in biodosimetry will allow for high
throughput and cost-effective assay automation in many readily accessible systems. In the present work,
we have utilized the commercial Cell::Explorer universal robot (Perkin Elmer) at Columbia Genome Center
to develope and optimize automated protocols for the dicentric and cytokinesis-block micronucleus
assays. Work supported by NIAID grant 5 U19-AI067773.
341 | P a g e
