Page 402 - 2014 Printable Abstract Book
P. 402
design. This includes logistic and ethics considerations for conducting large-scale molecular
epidemiological studies; logistics of biological sample collection, processing and storing; choice of
biomarker or bioassay; as well as awareness of potential confounding factors.
1
1
(SNF03) Telomere shortening in haemingioma cohort. Laure M. Sabatier ; Monika Frenzel ; Marion
3
1
3
2
Bellamy ; Aude Lenain ; Mohamed Amine Benadjaoud ; and Florent de Vathaire, Commissariat à
l’Energie Atomique (CEA), DSV/IRCM/SRO – Laboratory of Radiobiology and Oncology, Fontenay-aux-
2
1
roses, France ; 2Radiation Epidemiology Group, Villejuif, France ; and Radiation Epidemiology Group,
3
Villejuif, France
Cohorts allowing joint epidemiological and biological analyses are essential for direct radiation
risk assessment and radiation-induced cancers require accurate identification especially for dosimetry,
age at exposure, followed by post-irradiation. We present here data obtained with a cohort that has been
established comprising subjects irradiated (before age 1 year) for a skin hemangioma and followed at least
40 years. The doses of ionizing radiation received to the major organs were estimated below 100 mGy. In
this cohort, the overall incidence of cancer was 3 times higher than expected. Interplay between cellular
aging and the transmission of radiation induced damages could have consequences both directly in
“cancer” cells at different stage of the multistep carcinogenesis process or indirectly in stroma cells that
will lose the ability to keep dormant some cancer cells and will let them turn to in situ cancer and invasive
cancer. Besides the essential role of telomeres in cellular senescence and cancer development, exposure
to ionizing radiation may result in either telomere shortening or elongation depending on the given dose
and type of irradiation. Changes of the telomere length can occur homogeneous or heterogeneous for the
telomeres within one cell. To reveal the impact of the telomere status on genetic stability and on
individual susceptibility for getting cancer long-time after low dose irradiation we are performing an
expose/non-exposed study on a French Haemangioma cohort. We are analyzing the intra- and
intercellular (within one type of cell and between different cell types) heterogeneity of telomere length
of nucleated blood cells - especially of B- and T-lymphocytes - 40-60 years after treatment to assess the
potential for telomere-driven chromosomal instability even several years after exposure. First analysis
demonstrated that variations of the telomere length are dose dependent even for low doses below 100
mGy on the organ site (bone marrow). The analysis of chromosomal instability is underlining the
hypothesis that the effect of exposure to ionizing radiation is persistent and memorized for lifetime.
(SNF04) Radiation biomarker in papillary thyroid carcinoma. Horst Zitzelsberger; Martin Selmansberger;
Julia Hess; and Kristian Unger, Helmholtz Zentrum Muenchen, Neuherberg, Germany
A substantial increase in papillary thyroid carcinoma (PTC) among children exposed to the
radioiodine fallout has been one of the main consequences of the Chernobyl nuclear power plant accident
that occurred on 26 April 1986. Recently, the investigation of a PTC cohort consisting of young patients
that were exposed to the post-Chernobyl radioiodine fallout at very young age and a matched non-
exposed control group revealed a radiation-specific DNA copy number gain on chromosomal band
7q11.23 and the radiation-associated mRNA overexpression of the gene CLIP2, located on chromosome
7q11.23. We investigated the potential role of CLIP2 as a radiation marker for an individual classification
of PTCs into radiation-induced and sporadic cases. Thus, CLIP2 typing of PTCs allows its integration as a
epidemiological studies; logistics of biological sample collection, processing and storing; choice of
biomarker or bioassay; as well as awareness of potential confounding factors.
1
1
(SNF03) Telomere shortening in haemingioma cohort. Laure M. Sabatier ; Monika Frenzel ; Marion
3
1
3
2
Bellamy ; Aude Lenain ; Mohamed Amine Benadjaoud ; and Florent de Vathaire, Commissariat à
l’Energie Atomique (CEA), DSV/IRCM/SRO – Laboratory of Radiobiology and Oncology, Fontenay-aux-
2
1
roses, France ; 2Radiation Epidemiology Group, Villejuif, France ; and Radiation Epidemiology Group,
3
Villejuif, France
Cohorts allowing joint epidemiological and biological analyses are essential for direct radiation
risk assessment and radiation-induced cancers require accurate identification especially for dosimetry,
age at exposure, followed by post-irradiation. We present here data obtained with a cohort that has been
established comprising subjects irradiated (before age 1 year) for a skin hemangioma and followed at least
40 years. The doses of ionizing radiation received to the major organs were estimated below 100 mGy. In
this cohort, the overall incidence of cancer was 3 times higher than expected. Interplay between cellular
aging and the transmission of radiation induced damages could have consequences both directly in
“cancer” cells at different stage of the multistep carcinogenesis process or indirectly in stroma cells that
will lose the ability to keep dormant some cancer cells and will let them turn to in situ cancer and invasive
cancer. Besides the essential role of telomeres in cellular senescence and cancer development, exposure
to ionizing radiation may result in either telomere shortening or elongation depending on the given dose
and type of irradiation. Changes of the telomere length can occur homogeneous or heterogeneous for the
telomeres within one cell. To reveal the impact of the telomere status on genetic stability and on
individual susceptibility for getting cancer long-time after low dose irradiation we are performing an
expose/non-exposed study on a French Haemangioma cohort. We are analyzing the intra- and
intercellular (within one type of cell and between different cell types) heterogeneity of telomere length
of nucleated blood cells - especially of B- and T-lymphocytes - 40-60 years after treatment to assess the
potential for telomere-driven chromosomal instability even several years after exposure. First analysis
demonstrated that variations of the telomere length are dose dependent even for low doses below 100
mGy on the organ site (bone marrow). The analysis of chromosomal instability is underlining the
hypothesis that the effect of exposure to ionizing radiation is persistent and memorized for lifetime.
(SNF04) Radiation biomarker in papillary thyroid carcinoma. Horst Zitzelsberger; Martin Selmansberger;
Julia Hess; and Kristian Unger, Helmholtz Zentrum Muenchen, Neuherberg, Germany
A substantial increase in papillary thyroid carcinoma (PTC) among children exposed to the
radioiodine fallout has been one of the main consequences of the Chernobyl nuclear power plant accident
that occurred on 26 April 1986. Recently, the investigation of a PTC cohort consisting of young patients
that were exposed to the post-Chernobyl radioiodine fallout at very young age and a matched non-
exposed control group revealed a radiation-specific DNA copy number gain on chromosomal band
7q11.23 and the radiation-associated mRNA overexpression of the gene CLIP2, located on chromosome
7q11.23. We investigated the potential role of CLIP2 as a radiation marker for an individual classification
of PTCs into radiation-induced and sporadic cases. Thus, CLIP2 typing of PTCs allows its integration as a
