Page 6 - BIO REX PAPAYA English

strict temperature control and oxygen supply (continuous

mixing with optimal speed), which allows to obtain highly

standardised final product with desired/designed properties.

The temperature is maintained at 25±0.2°C during all duration

of the fermentation process, the stirring is carried out at 15

cycles/min, and the air circulation takes place through close

cycle tubes of the bioreactor. The entire process takes 180

days.

The process of fermentation is frequently in-process

controlled for microbial contamination to carry out strict

quality control. The final product is pressed, liquid supernatant

is collected, sterilized, and filtered through microbial filters.

The essential step of sterilization is performed under low

temperature (55°C) that allows the product to be free of

contaminants, and at the same time preserves temperature

unstable substances, such as plant polyphenols.

The qualitative/semi-quantitative analyses of the final product

consisting of a mixture of bio-active substances are carried out

using HPLC, MS-GC, spectrophotometry, and fluorescent

methods. Also, the antioxidant and free radical scavenging

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