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Instead, a gradual ramp-up followed by maintenance dosing is proving more effective and biologically

aligned. This allows the body to integrate cellular signals, modulate inflammation, and enhance healing
without triggering an overactive immune response. The body is inherently programmed for balance
(homeostasis), and regenerative processes must operate within physiological limits. Emerging data
indicate that gradual introduction and ramping up of MSC-derived products—especially exosomes—
leads to more sustained benefits, allowing:

In animal models (including rodent studies), incremental administration of exosomes showed better
regenerative outcomes than single, high-dose applications. This aligns with the understanding that
bioactive signals are more effective when the body has time to respond and recalibrate.

WHY FRESH, PURE MSCS WORK BETTER IN SMALLER DOSES

Fresh, Pure MSCs: Potent at Lower Doses

Our approach mirrors natural biological rhythms—introduce gently, build progressively, and maintained as needed.
Our fresh MSC formulations (ranging from 10 to 80 million cells per application) are:

✓ None Frozen, Fresh-Live Preservative Free
✓ 95%-100% Cell Viability, making them biologically potent and immediately functional upon
administration
✓ High quality sourcing and lab standards ensure strong homing, signaling and regenerative
behavior-without needing excessive quantity.

EXOSOMES: SIGNALING REQUIRES BALANCE

Like MSCs, MSC-derived exosomes carry molecular messages that trigger healing and immune
modulation. Research in animal models shows that dosing in stages—gradual introduction, build-up, and
tapering—leads to better tissue response and longer-lasting outcomes. High exosome doses all at once
may desensitize receptor pathways or dampen therapeutic signals.

A Balanced Biological Response: The body is inherently programmed for balance (homeostasis),

and regenerative processes must operate within physiological limits. Emerging data indicate that gradual
introduction and ramping up of MSC-derived products—especially exosomes—leads to more
sustained benefits, allowing:
• Optimal cell-to-cell communication
• Controlled anti-inflammatory signaling

• Enhanced tissue responsiveness and integration

CONTROLLED DOSING - SUPPORTING RESEARCH

❖ Caplan AI (2020) emphasized that MSCs primarily function via their secretome, and excessive
doses can disrupt normal signaling cascades. MSCs act primarily via paracrine signaling, not
cell replacement; excessive dosing can interfere with this

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