Page 4 - Gastric pentadecapeptide BPC 157

M. Sture.tinic cpl ul. / Jourtiul of Orthupuerlic Riwurch 21 (2003) 976-983 979
Table I
Rat Achilles tendon transection
Assessed Therapy Time after Achilles tendon transection
parameter
Day 4 Day 7 Day 10 Day 14
Med Min Max Med Min Max Med Min Max Med Min Max
~~
Failure Healthy 58.30 47.22 67.29 61.28 47.22 67.29 75.20 58.00 83 10 63.50 58.30 97.27
load Saline ____ 12.781 27.710 15.637 39.258 is.526 7o.300 68.261
3.223
9.119
10 Pg 8.2556.892 13.098 30.701 26.513 36.084 56.445 43.945 71.094 63.500 58.301 97.266
10 ng 10.406 6.641 15.136 30.591 17.053 36.474 50.732 45.556 65.723 68.408 57.617 88.281
10 P6 ~~ 10.821 2.673 14.013 31.762 25.879 43.774 47.412 26.196 61.474 73.096
Failure Healthy 11.28 8.99 12.82 11.28 8.99 12.82 13.50 12.60 15.00 12 10 1 I 10 18.53
load per Saline ____ 2.434 5.278 2.979 10.668 7.338 13.390 9952 6678 13.002
1.737
0.614
1.314
1.573
area 10 Pg ____ 2.495 5.848 5.050 6.873 10.751 8.371 13.542 12.095 11.105 18.527
10 ng ____ 2.883 5.827 3.248 w 9.663 8.677 12.519 13.030 10.975 16.815
1.265
1.982
0.509
2.061
10 Pg ____ 2.669 6.05 4.929 9.031 4.990 11.709 4567 13.923
Young’s Healthy 10.44 5.77 13.90 7.69 5.66 13.90 11.80 11.30 13.90 10 70 5 66 15.08
modulus of Saline 2.2800.402 2.988 4.811 3.337 5.925 5.158 9.877 4454 8.795
elasticity 10 Pg _ 1.030 3.958 ~ 5.333 3.722 7.183 7.887 $.771 17.632 10.702 5.662 15.077
1.948
_
10 ng 2.937 _ 1.866 _ 3.758 5.723 2.034 6.395 4.206 8.875 m 5645 13.509
~
_
10 Pg 2.3310.855 3.775 4.921 3.446 6.049 5.974 3.552 7.791 6309 4.199 9.290
Fibroblast Saline 1326 1056 I430 1770 1626 I920 1976 1872 2100 I884 1626 2172
number 10 Pg 1420 1350 1480 2014 I988 2097 2400 2100 2556 2196 1818 2460
10 ng 1377 1067 1563 2053 1889 2106 2266 2051 2456 1870 1670 1956
10 Pg 1255 1107 1580 I693 1623 1886 1934 1806 2106 1837 1677 2043
Reticulin Saline 0.350 0.320 0.370 ~ - - - -
proportion 10 Pg 0.540 0.500 0.590 - - - - - -
10 ng 0.520 0.500 0.530 - - - - - - - ~
10 Pg 0.360 0.320 0.380 - - - - -
Collagen Saline - - - 0.24 0.19 0.29 0.321 0.254 0.367 0 396 0 356 0. 440
proportion 10 Pg - - 0.40 0.30 0.46 0.506 0.485 0.520 0.521 0.465 0.610
10 ng - 0.38 0.28 0.48 0.480 0.465 0.510 0.502 0.43 7 0.589
10 Pg - 0.25 0.19 0.30 0.325 0.289 0.356 0 422 0.337 0.438
Gape Saline 6.4 5.6 7.8 2.95 2.2 2.8 0.15 0 0.5 01 0 0.2
between the 10 LIE 2.6 2.0 3.2 0.05 0 0.2 0.2 0 0.6 01 0 0.2
cut ends 10 ng 2.6 2.0 3.6 0. I 0 0.3 0.1 0 0.2 01 0 1
.-
10 P?z 6.7 5.0 7.6 3.1 2.5 30 0.1 0 0.2 01 0 0.2
Assessment at post-operative day 4, 7, 10, 14: (ij biomechanical (failure load (N), failure load per area (Nlmm’j, Young’s modulus of elasticity). (ii)
microscopy (reticulin proportion, fibroblast number, collagen proportion per visual field), (iii) macroscopy (size of the defect (longest diameter. mm)
in tendon between the cut ends. Agents (/kg b.w., i.p., once time daily) (gastric pentadecapeptide BPC 157 (10 pg, 10 ng or 10 pg) or saline (5.0 ml)).
were firstly applied at 30 min after surgery, the last application at 24 h before autopsy. Median/Minimum/Maximum (Med/Min/Max). Kruskall-
Wallis and Mann-Whitney tests (due to Bonferroni correction, the values ofp < 0.016 or less were considered to be statistically relevant. vs. controls
with tvansccted tendon. or GS. normal healthy rats). (-) not assessed.
growth stimulation (Fig. 5). Adding BPC 157 after HNE cpm/well [3630-36901 vs. 2500 cpm/well [2487-25121,
results in a growth stimulation, while the peptide abol- p = 0.021).
ished inhibiting effect of HNE.
Discussion
Serurn deprived conditions
TGF-PI strongly increased cultured tendocytes DNA Stable peptide can play a major role in generating
synthesis, while BPC 157 itself did not change the therapy for tendon healing and this study focus was on a
growth of the cells (Fig. 6). stable gastric pentadecapeptide BPC 157. Currently in
HNE alone showed a slight inhibiting activity (me- clinical trials for inflammatory bowel disease (PLD-116,
dian [minimum-maximum]) (485 cpm/well [470490]) PL- 14736, Pliva), it ameliorates internal and external
compared to HNE administered after BPC 157 (507 wound healing [8,12,16,17,19-21,31]. Here, systemic
cpm/well [499-512]), p = 0.021. administration-improvement of an injured tendon fol-
Combined with TGF-Pl HNE added after TGF-P1 lows from a general evidence presenting this tendon
resulted in more pronounced growth inhibition (3647 repair similar to the repair of other connective tissues