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1430 uMSC Exosomes Suppress Scar Formation

Figure 3. Themyofibroblast-suppressingabilityofuMSC-ExosmainlydependsonitsRNAcomponents.(A):Gelelectrophoresis(left)showingtheRNase
digestedexosomesweredepletedofRNAscomparedwithproteinaseandcontroltreatment.Silverstaining(right)showingthatafterproteinasetreatment,
exosomes were degraded thoroughly. (B): NanoSight analysis showing that proteinase treatment did not compromise exosome integrity (additional data
shown in supplemental online Fig. 3). (C): Quantitative reverse transcription-polymerase chain reaction showing a-SMA and collagen I expression in the
indicatedenzymedigestiongroups.Dataarepresentedasmean6SD;n=3;pp,p,.01.(D):WesternblotanalysisshowingthatRNase-treatedexosomes
cannotdownregulatetheproteinlevelofSMAinducedbyTGF-b.(E):Collagengelcontractionassayassessingthecontractioninhibitoryeffectofdifferent
enzyme-digested exosomes showing representative images (left) and measurement of gel diameter (right). Data are presented as mean 6 SD;
n =3; pp, p , .01. (F): Cell cycle analysis of different enzyme-treated exosomes showing representative images (left) and percentage of G 2
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