Page 53 - PC 101 practical notes 24-25..

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL I PHARM. ANAL. CHEM. I (PC 101)

C Ca al lc cu ul la at ti io on ns s : :

E.P 1 (M.O. reading) ≡ HCl only

E.P 2 (ph.ph. reading) ≡ HCl + CH 3COOH
 (E.P 2 - E.P 1) ≡ CH 3COOH only
For HCl :

⎯
HCl + NaOH ⎯→ NaCl + H 20
 1 NaOH ≡ 1 HCl
standard sample
Equivalence factor (F):

1 x M.W. of HCl x 0.1
each ml of 0.1 N NaOH ≡ ≡ .......... g HCl
1 x 1000

Concentration:

E.P 1 x F x 1000
Concn. of HCl = = .......... g/L
10

For CH 3COOH :

⎯
CH 3COOH + NaOH ⎯→ CH 3COONa + H 20
 1 NaOH ≡ 1 CH 3COOH
standard sample
Equivalence factor (F):
1 x M.W. of CH 3COOH x 0.1
each ml of 0.1 N NaOH ≡ ≡ ..... g CH 3COOH
1 x 1000
Concentration:

(E.P 2 - E.P 1) x F x 1000
Concn. of CH 3COOH = = .......... g/L
10

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