Page 266 - ebook HCC

PROGRAMME
264 PROGRAMME AND ABSTRACTSAND ABSTRACTS GENEVA, SWITZERLAND EASL HCC SUMMIT 265
264
FEBRUARY 13 - 16, 2014

Poster Board Number C45

MOLECULAR ANALYSIS OF THE GENE GSTP1 NOTES
ALA114VAL POLYMORPHISM IN PATIENTS
WITH CIRRHOSIS AND HEPATOCELLULAR
CARCINOMA (HCC)

Gislaine D. Ferreira , Pamela R. Francelin , Renato F. Da Silva ,
1
1
2
Rita D. C. M. A. da Silva , Ana Lívia S. Galbiatti , Anelise Russo , Vivian R. Corea ,
1
1
1
1
Paulo C. Arroyo Júnior , William J. Duca , André R. de Oliveira , Leonardo P. Stuchi ,
1
2
1
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Helen C. de Felício , Érika C. Pavarino , Eny M. G. Bertolo 1
1
3
2
1 Clinical Department, General Surgery, Nurse Department, Faculty of Medicine of São
3
José do Rio Preto, São José do Rio Preto, Brazil
Corresponding author’s e-mail: renatosilva@famerp.br
Introduction: Hepatocellular carcinoma (HCC), epithelial tumor derived from hepatocytes,
is rated as the most frequent primary tumor of the liver. Observed genetic variation within
and between populations have been postulated as an influence on the risk factors for
HCC. Currently, the most studied are genetic variants of glutathione S-transferase (GST).
Aims: To analyze GSTP1 Ala114Val polymorphism in patients with cirrhosis and
hepatocellular carcinoma and in individuals with no history of cancer (control group), in
order to identify biomarkers of susceptibility for HCC.
Methodology: We included 284 individuals (84 patients and 200 controls). The etiology
of cirrhosis were: HCV= 35; HBV= 10; HCV and alcohol=26; alcohol=50. Molecular
analysis was performed by Polymerase Chain Reaction – Restriction Fragment Length
Polymorphism PCR-RFLP. The studied variables were: age, gender, alcohol consumption,
CLINICAL POSTER ABSTRACTS Results: The results showed that age ≥ 42 years (OR 18.33, 95% CI 6.01 to 55.91, p = CLINICAL POSTER ABSTRACTS
virus C infection and cirrhosis. For statistical analysis, the chi-square and multiple logistic
regression tests were adopted, with p ≤ 0.05 and 95% CI considered significant.
0.000), female gender (OR 0.20, 95% CI 0.07 to 0.54 p = 0.001), alcohol consumption (OR
3.89, 95% CI 1.64 to 9.23, p = 0.002) and the presence of GSTP1 Ala114Val polymorphism
(OR 5.26, 95% CI 1.64 to 16, 87, p = 0.005) are risk factors for developing cirrhosis
and hepatocellular carcinoma. Virus C infection in conjunction with alcohol consumption
(OR 66.76, 95% CI 1.16 to 3829.18, p = 0.042) increases the chance of development of
cirrhosis for hepatocellular carcinoma.
Conclusion: Individuals aged ≥ 42 years old, female gender, alcohol consumption
and presence of the GSTP1 Ala114Val polymorphism may be predictive factors for the
development of cirrhosis and hepatocellular carcinoma. Virus C infection in conjunction
with alcohol consumption can further increase the chance of development of cirrhosis for
hepatocellular carcinoma.