Page 5 - BUKU AJAR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Buku Ajar High Performance Liquid Chromatography (HPLC)

DAFTAR ISI

Contents

PRAKATA .................................................................................................................. ii
DAFTAR ISI .............................................................................................................. iii
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)................ 1
A. Kompetensi ...................................................................................... 1

B. Pendahuluan .................................................................................... 1

1. Sejarah Perkembangan HPLC ................................................................. 2

2. Jenis-jenis Kromatografi ........................................................................ 4
a. Liquid Liquid Chromatography (LLC) ..................................................................... 5
b. Liquid Solid Chromatography (LSC) ...................................................................... 5
c. Ion-exchange chromatography ............................................................................ 5
d. Exclusion chromatography ................................................................................... 6
Latihan ........................................................................................................................ 6

C. Prinsip Teoritis High Performance Liquid Chromatography (HPLC) ..... 7

1. Prinsip Pemisahan HPLC ........................................................................ 8

2. Pelebaran Pita dan Optimasi Kolom ..................................................... 17
3. Optimasi Kinerja Kolom ....................................................................... 25

D. Instrumentasi HPLC ....................................................................... 28

1. Reservoir pelarut ................................................................................. 28

2. Pompa ................................................................................................. 30
3. Injektor ............................................................................................... 32

4. Kolom kromatografi ............................................................................. 33

5. Fase Diam ............................................................................................ 36
6. Fase Gerak ........................................................................................... 37

7. Penghubung tubing dan fitting ............................................................. 39

8. Detektor .............................................................................................. 39
9. Perangkat pengumpul data .................................................................. 46

E. Aplikasi HPLC ................................................................................. 47

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