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Kinetic Multi-Spheroid Assays
IncuCyte S3 multi-spheroid automated image acquisition and Segmented DF Brightfield images compare vehicle or CMP treated
analysis software tools enable label-free, kinetic quantification of conditions at 168 h. Time courses show the individual well total
growing and shrinking multi-spheroids over time. Figure 4 (below) Brightfield Object area (μm2) (y-axis) over 168 h and illustrate
illustrates the growth curves of three multi-spheroid tumor models specific cell type-dependent kinetic profile of spheroid growth
over several days and demonstrates the inhibitory effects of the and shrinkage. Data were collected over 168 hour period at 6 hour
cytotoxic drug camptothecin (CMP) on their growth trajectories. intervals. All images captured at 10x magnification. Each data
point represents mean ± SEM, n=6 wells.
Vehicle CMP (1µM)
A549
MCF-7
MDA-MB-231
Figure 4. Real-time quantification of the growth of A549, MCF-7 and MDA-MB-231 spheroids. Cells were seeded in flat-bottom 96-well plates (2,000 cells
per well) on a bed of Matrigel® and spheroids allowed to form (72 h). Spheroids were treated with either vehicle (0.1% DMSO) or CMP (10 μM).
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