Page 83 - Live-cellanalysis handbook

Kinetic Assays Utilizing Complex Models

Shortcomings of Traditional Assays Live-Cell Imaging and Analysis Approaches

• Endpoint collection of data misses • Continuous long term monitoring captures important kinetic information on
important information between imaging cellular processes and morphology, providing for additional mechanistic details.
intervals that is needed for complete • Uninterrupted environmental control within incubator, coupled with automated
insight into biological processes.
image acquisition (DF Brightfield, HD Phase, and fluorescent) results in a continuous
• Repeated removal of cells from incubator physiologically-relevant environment.
for lengthy image acquisition results in loss
of control for O and CO and multiple • Label-free analysis of growth and shrinkage.
2
2
environmental fluctuations. • Non-perturbing, optimized, validated cell health reagents for enhanced physiological
• Incorporation of labels that can perturb relevance.
cells and may confound interpretation. • Automated acquisition and analysis of high quality, high contrast images, combined
• Additional time required for development with lab-tested protocols, to quickly gather reproducible, quantitative data for
and optimization of image acquisition pharmacological screening in 96 and 384-well formats.
parameters. • Purpose built software tools and guided interface enables non-expert operators to
• Complex image processing requires perform image processing and generate publication-ready graphics.
expert operation and training for data
generation and analysis.

Table 1. Traditional vs Live-cell Analysis.

How Live-Cell Spheroid Assays Work

Multi-spheroid assays Single spheroid assays

Scaffold-based, multi-spheroid assays allow for study of cell These assays provide an integrated solution to automatically
cultures in a more physiologically relevant, 3D format. These track and quantify single tumor spheroid formation, growth, and
assays permit the tracking and quantification of spheroid health in real time with minimal environmental disturbance inside
formation, cell-cell, and cell-ECM interactions in real time inside a tissue culture incubator, providing additional physiological
a tissue culture incubator. insights.

81