Page 80 - Live-cellanalysis handbook
P. 80

Live-Cell Analysis Handbook — Third Edition


       Conclusions

       IncuCyte® live-cell Immunocytochemistry demonstrates how   •    Proximity relationships, such as those between the tumor and
       traditional ICC can be extended from a traditional ‘fix and stain’   immune cells presented here, can be observed and quantified at
       approach to a method that allows for the dynamic monitoring of   throughput with unprecedented accessibility.
       surface proteins in living cells over several days. Using a simple   •    IncuCyte® FabFluor-488 antibody labeling reagent is specific,
       and robust labeling approach, IncuCyte® FabFluor-488 labeling   stable under physiological conditions for long incubations, and
       reagents in conjunction with the IncuCyte® Live-Cell Analysis   allows image analysis without disturbing the biology, health or
       System, enables real-time quantification of dynamic changes in   proliferation of cells.
       protein abundance and distribution with the ability to associate
       changes in cell morphology, function as well as study complex   •    The reagent has been used successfully with a range of
       cell interactions.                                       antibodies in a variety of cells displaying surface proteins –
                                                                including immune cells, nerve cells and tumor cells.
       •    Live-cell ICC can reveal time-and concentration-dependent   •    The reagent removes the need for researchers to source
         changes in cell surface, and can be combined with analyses   antibodies with appropriate fluorescent tags for their molecule
         of other morphological and phenotypic parameters to link   of interest.
         surface protein expression changes with cell function over time
         proteins.                                            •    The live-cell ICC method saves time – assays leverage
                                                                automated acquisition and analysis of data and can be carried
       •    By labeling different cell surface markers with IncuCyte®   out simultaneously in up to six 384-well plates, without
         FabFluor-488 reagent it becomes possible to track the   laborious fixation and washing protocols.
         movement and interaction of subsets of living cells, revealing
         the dynamics of their interactions.






















































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