Page 27 - Differential Diagnosis in Small Animal Cytology, The Skin and Subcutis
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Chapter 2
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Fig. 2.3. Squash smear preparation. Fig. 2.4. Blood smear technique.
Line smear technique
• Use
Appropriate for hypocellular fluids.
• Technique
The procedure is similar to the blood smear technique, but instead of completing the smear,
the spreader slide is lifted vertically prior to formation of a feathered edge. This results in
concentration of cells along a ‘line’ where the smear is interrupted.
Centrifugation
• Use
Appropriate for cytology preparation of hypocellular fluids.
• Technique
Concentration may be obtained by cytocentrifugation or traditional low-speed centrifuga-
tion. The latter requires a drop of the sediment to be transferred on to a slide and then
smeared with either the squash or blood smear technique, depending on its density.
