Page 911 - The Toxicology of Fishes

The Effects of Polycyclic Aromatic Hydrocarbons in Fish from Puget Sound, Washington 891

Eagle Harbor
City Wwy
Elliott Bay Outside
Port Gardner-Everett
Hylebos Wwy
Mukilteo
Elliott Bay Waterfront
Duwamish Wwy
Commencement Wwys
Sinclair Inlet
President Pt
Budd Inlet
Browns Pt
Edmonds
Commencement Outside
Case Inlet
Richmond Beach
Port Susan
Port Madison
Carkeek Park
Useless Bay
Alki Pt
Clinton
Discovery Bay
Port Gardner-Sno. River
10 100 1000 10000 100000 1000000
Sediment TAHs (ng/g dry wt.)
FIGURE 22.7 Concentrations of aromatic hydrocarbons (ng/g dry weight) in sediments from sites in Puget Sound. (From
Johnson, L.L. et al., Can. Tech. Rep. Fish. Aquat. Sci., 1948, 304–329, 1994. With permission.)

Eagle Harbor Inside (30)
Duwamish Wwy, EB (58)
Hylebos Wwy, CB (20)
Everett Harbor (37)
Polnell Point (24)
Eagle Harbor Outside (12)
Richmond Beach (21)
Everett Harbor Outside (17)
West Point, EB (20)
Pilot Point (22)
Useless Bay (16)
Carkeek Park (18)
President Pt. (20)
Edmonds (21)
0 1000 2000 3000
Biliary FACs-BaP (ng/g bile)
FIGURE 22.8 Concentrations of fluorescent aromatic compounds (FACs) in bile (ng/g bile) of English sole from Puget
Sound. (From Johnson, L.L. et al., Can. Tech. Rep. Fish. Aquat. Sci., 1948, 304–329, 1994. With permission.)

Fish from contaminated areas show not only elevated levels of FACs in bile but also other physiological
changes that are indicators of biological responses to contaminant exposure, including increased AHH
activity and covalent binding of PAHs to DNA in liver. Both of these types of biochemical alteration are
observed in English sole, starry flounder (Platichthys stellatus), and rock sole (Pleuronectes bilineata)
from areas in Puget Sound with elevated sediment PAH concentrations (Collier and Varanasi, 1991; Collier
et al., 1995; Stein et al., 1992) (Figure 22.9). Moreover, both hepatic AHH activity and levels of DNA
adducts in liver, determined by P-postlabeling, are significantly correlated with sediment PAH concen-
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trations at sites where fish are collected, and with biliary FAC concentrations in the same fish (Stein et