Page 1028 - Veterinary Immunology, 10th Edition

immediately. It is important to maintain urine flow with fluids and
VetBooks.ir a diuretic because hemoglobin may cause renal tubular destruction.

Recovery follows elimination of the foreign red cells.
Transfusion reactions can be almost totally prevented by prior

testing of the recipient's serum for antibodies against the donor's
red cells. The test is called cross-matching. Blood from the donor is
centrifuged and the plasma discarded. The red cells are then
resuspended in saline and recentrifuged. This washing procedure is

repeated (usually three times), and eventually a 2% to 4%
suspension of red cells in saline is made. These donor red cells are
mixed with recipient serum and incubated at 37° C for 15 to 30
minutes. If the donor red cells are lysed or agglutinated by the

recipient's serum, no transfusion should be attempted with those
cells. It is occasionally found that the donor's serum may react with
the recipient's red cells. This is not of major clinical significance
because transfused donor antibodies are rapidly diluted within the

recipient. Nevertheless, blood giving such a reaction is best
avoided.

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