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104                Natural Antioxidants: Applications in Foods of Animal Origin
  VetBooks.ir  strong antioxidant. The total polyphenol content of bearberry extract was


            reported by Carpenter et al. (2007) as 57.4 g ± 1.73 GAE/100 g. Bearberry
            extract reduced the lipid oxidation in raw and cooked pork patties during
            storage up to 12 days at 4 °C and sensory properties were affected by its
            addition. Pegg et al. (2005) had reported that bearberry leaf extract possesses
            marked antioxidant activity in model and meat systems. Crude leaf extract,
            and its fractions (acetone, ethanol) inhibited TBARS formation in cooked
            meat systems after seven days of refrigerated storage.



            3.5.1.3  CAROB FRUIT

            The Carob is the fruit of an evergreen Ceratonia silique L. cultivated in the
            Mediterranean area. Use of the whole carob fruit for consumption is rather
            limited, due to a high level of tannins causing astringency (Avallone et al.,
            1997). The two main carob pod constituents are pulp (90%) and seed (10%).
            The seed coat contains antioxidants (Batista et al., 1996). Phenolic contents
            of pulps and leaves from carob tree have been reported (Avallone et al.,
            1997; Corsi et al., 2002; Kumazawa et al., 2002; Owen et al., 2003; Makris
            & Kefalas, 2004). Flavonol glycoside, 4`-p-hydroxybenzoylisorhamnetin-
            3-O-α-L-rhamnopyranoside  named  ceratoside,  together  with  the  known
            kaempferol-3-O- α-L-rhamnopyranoside (afzelin), quercetin-3-O- α -L-arabi-
            nofuranoside (auriculain), quercetin-3-O- α-L-rhamnopyranoside, β-sitosterol,
            and β-sitosterol-3-O- β -D-glucoside were isolated from carob seeds (Gohar
            et al., 2009). Vaya and Mahmood (2006) observed that the carob leaves are
            rich in flavonoids; and more than nine compounds were identified. Researchers
            had isolated and identified the major polyphenols in carob fibers (Owen et al.,
            2003; Papagiannopoulos et al., 2004).
               Bastida et al. (2009) evaluated the effect of adding condensed tannins
            in the form of non-purified (Liposterine ) or purified (Exxenterol ) extracts
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            obtained from carob fruit to prevent oxidation in lipid-cooked pork meat
            systems during chilling and frozen storage. The antioxidant activity of these
            extracts was compared with that of α-tocopherol (TM). TBARS levels were
            significantly  lower  in  samples  containing  Liposterine  (LM),  Exxenterol
            (EM), and TM than in control sample under chilled storage. TBARS forma-
            tion was similar (P > 0.05) for LM and EM but significantly lower than that
            for TM. Thermal oxidation compounds were lower (P < 0.05) in EM than in
            LM or TM, which is also having nutritional importance as thermal oxidation
            products are potentially toxic. Therefore, Carob extract has the potential to
            improve the fat stability and toxicological safety of meat systems.
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