Page 600 - Veterinary Immunology, 10th Edition

complexed to magnetic beads. The residual cells can be cultured in
VetBooks.ir the presence of IL-2, and IL-15. The non-T, non-B large granular

cells that survive are NK cells (IL-2 is necessary for survival, IL-15 is
+
−
needed for expansion). These NK cells are CD3 , granzyme B ,
+
+
CD45 , and MHC class I . Both IL-15 and IL-21 stimulate their
proliferation, receptor expression, and cytotoxic functions. They can
lyse distemper-infected target cells as well as cancer cells from
thyroid adenocarcinomas, melanomas, osteosarcomas, and

mammary carcinomas.

Cats

Feline NK cells are large granular lymphocytes found in the blood
and spleen. In general, cat NK cell numbers and properties are

similar to those in other domestic mammals. However, unlike other
+
species, 10% to 30% of feline NK cells are CD11b and selectin
+
CD62L . They are active against feline target cells infected with
feline leukemia virus, herpesvirus, or vaccinia virus.

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