Page 204 - The Manga Guide to Biochemistry

Take a look at this graph. When We did it!
the x-axis value is 0, the y-axis We got Vmax!
value is approximately 0.019. That
means that, in this reaction, the
Vmax value of DNA polymerase is

1/0.019, or about 52.6.

In other words, the
DNA polymerase can
react with nucleotides
to synthesize about
52.6 pmol of new DNA*

per hour.**

Reciprocal of Slope
reaction rate (pMol/hour)

Reciprocal of substrate
concentration (1/µM)

Hang on, Kumi. You’re If you look carefully at
celebrating a bit too this reciprocal plot, you
soon. Remember, this
can see that the slope
graph also lets us of the straight line is
calculate the exact
represented by
value of Km!

I hope you
remember your
algebra because

we’re diving
right in!

* This value can vary according to the measurement conditions or the type of DNA polymerase. 52.6 pMol/hour
is just an example.

** Remember, the DNA polymerase reaction time was 60 minutes.