Page 205 - The Manga Guide to Biochemistry
P. 205
Let’s use the Michaelis-Menten equation to figure out Km and Vmax!
v = Vmax [S]0 Let’s do it!
[S]0 + Km
1
If we take the reciprocal v , we get...
1 = [S]0 + Km = [S]0 + Km
v Vmax [S]0 Vmax [S]0 Vmax [S]0
= 1 + Km × 1
Vmax Vmax [S]0
In other words, we can draw the graph of the straight line y = ax + b, where y : 1
v
y x: 1
[S]0
Slope a a : Km
Vmax
b: 1
Vmax
x
A linear function? I That’s right! The graph
learned about those of a linear function is
in middle school. No just a straight line, so
this makes our job much
problem! (I think...)
easier because...
No matter how far
we extend the line,
our formula will
always work!
Enzymes Are the Keys to Chemical Reactions 191
