Page 23 - Phytochemistry -1 (PG404) / Clinical Pharmacy 2nd level students ( 2019 )

Clinical pharmacy PharmD program Third level Phytochemistry-1 (PG-504)

Pb subacetate white precipitate No white -ve
precipitate

Borax Precipitate -ve -ve

Oxidase enzyme:

Aqueous solution + hydrogen peroxide + few drops of benzidine ; shake
; warm at 37°C.

If produce blue color or greenish blue, Oxidase enzyme is present,,,

So it is gum Arabic (gum acacia).

B. Mucilage

1. Agar - Agar

* It consists of Ca salt of sulfated polysaccharides.

* It can be resolved into 2 parts:

Agarose: It is the principle fraction responsible for the strength of agar.

Agaropectin: It is sulfated galactose.

* Insoluble in cold water, Soluble in hot water.

Tests for identification

Boil 1 gm agar power in water to form gell, Then divide the sol. into
different fraction to apply the followings reaction:

1. Cooling Stiff gel.

2. I₂ Reddish Particles.

3. Hcl + BaCl₂ White precipitate.

4. HCl/heat; neutralize Reduction (red precipitate) due to
+ Fehling test galactose.

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