Page 34 - Phytochemistry -1 (PG404) / Clinical Pharmacy 2nd level students ( 2019 )
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Clinical pharmacy PharmD program Third level Phytochemistry-1 (PG-504)
1- Run the iodometric method for the mixture solution as before to get an
exact end point which equivalent to glucose only (EP 1)
2- Run the copper reduction method for the mixture solution as before to
get an exact E.P (E.P 2) which is equivalent to total reducing sugar.
Calculations
(Blank – EP 1) X 0.009 X 100
% glucose = From Iodometry =
= A = gm % glucose
25
% total reducing sugars = glucose + fructose = from fehling
0.05 X 100
= = B = gm %
E.P 2 – 0.2
% Fructose = B - A
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